This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon. Fusion transcripts, which combine part of this gene with the 5' exons of the neighboring POC1B (POC1 centriolar protein homolog B) gene, also exist. [provided by RefSeq, Dec 2010]
Transcription factors with Perturb-seq knockdown data for GALNT4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GALNT4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GALNT4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:88,578,197–88,581,128 | 944.2 kb | Distal (>10kb) Multiome HiCAR | 819 | |
| chr12:89,225,440–89,226,374 | 298.8 kb | Distal (>10kb) Multiome | 416 | |
| chr12:89,247,539–89,248,148 | 277.0 kb | Distal (>10kb) Multiome | 291 | |
| chr12:89,344,903–89,347,295 | 178.9 kb | Distal (>10kb) Multiome | 690 | |
| chr12:89,349,881–89,350,937 | 174.6 kb | Distal (>10kb) Multiome | 360 | |
| chr12:89,351,407–89,355,035 | 170.9 kb | Distal (>10kb) Multiome HiCAR | 1222 | |
| chr12:89,411,690–89,412,513 | 112.7 kb | Distal (>10kb) Multiome | 89 | |
| chr12:89,524,103–89,526,498 | 1.3 kb | Proximal (<10kb) Multiome | 1007 | |
| chr12:89,529,864–89,530,021 | 5.1 kb | Proximal (<10kb) | 103 | |
| chr12:89,707,516–89,710,690 | 184.8 kb | Distal (>10kb) Multiome | 1065 | |
| chr12:89,777,305–89,778,322 | 253.0 kb | Distal (>10kb) Multiome | 135 | |
| chr12:90,953,653–90,954,801 | 1429.3 kb | Distal (>10kb) Multiome HiCAR | 264 |
Genomic view of the GALNT4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.