Transcription factors with Perturb-seq knockdown data for FZD4-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FZD4-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FZD4-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:86,799,611–86,801,237 | 155.2 kb | Distal (>10kb) Multiome | 470 | |
| chr11:86,954,420–86,956,821 | 9 bp | At TSS Multiome | 987 | |
| chr11:86,996,771–86,997,659 | 41.5 kb | Distal (>10kb) Multiome | 144 | |
| chr11:86,999,558–87,000,730 | 44.8 kb | Distal (>10kb) Multiome | 620 | |
| chr11:87,037,068–87,038,477 | 82.2 kb | Distal (>10kb) Multiome | 959 |
Genomic view of the FZD4-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.