FOXO3
forkhead box O3 | AF6q21, FOXO2, FKHRL1, FOXO3A

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Member of: DE-4 DE-4.2
Biological processes 98 terms
DNA binding (GO:0003677)DNA binding (GO:0003677)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227)DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)beta-catenin binding (GO:0008013)beta-catenin binding (GO:0008013)cellular response to amyloid-beta (GO:1904646)cellular response to corticosterone stimulus (GO:0071386)cellular response to glucose starvation (GO:0042149)cellular response to glucose stimulus (GO:0071333)cellular response to hypoxia (GO:0071456)cellular response to nerve growth factor stimulus (GO:1990090)cellular response to oxidative stress (GO:0034599)cellular response to oxidative stress (GO:0034599)chromatin (GO:0000785)chromatin DNA binding (GO:0031490)chromatin DNA binding (GO:0031490)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)membrane (GO:0016020)mitochondrial matrix (GO:0005759)mitochondrial matrix (GO:0005759)mitochondrial matrix (GO:0005759)mitochondrial matrix (GO:0005759)mitochondrial matrix (GO:0005759)mitochondrial outer membrane (GO:0005741)mitochondrial outer membrane (GO:0005741)mitochondrial transcription (GO:0006390)mitochondrial transcription (GO:0006390)mitochondrial transcription factor activity (GO:0034246)mitochondrial transcription factor activity (GO:0034246)mitochondrion (GO:0005739)mitochondrion (GO:0005739)negative regulation of cell migration (GO:0030336)negative regulation of neuron differentiation (GO:0045665)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of DNA-templated transcription (GO:0045893)positive regulation of apoptotic process (GO:0043065)positive regulation of autophagy (GO:0010508)positive regulation of autophagy (GO:0010508)positive regulation of endothelial cell apoptotic process (GO:2000353)positive regulation of erythrocyte differentiation (GO:0045648)positive regulation of hydrogen peroxide-mediated programmed cell death (GO:1901300)positive regulation of miRNA transcription (GO:1902895)positive regulation of miRNA transcription (GO:1902895)positive regulation of muscle atrophy (GO:0014737)positive regulation of muscle atrophy (GO:0014737)positive regulation of neuron apoptotic process (GO:0043525)positive regulation of reactive oxygen species biosynthetic process (GO:1903428)positive regulation of regulatory T cell differentiation (GO:0045591)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)protein kinase binding (GO:0019901)protein-containing complex (GO:0032991)protein-containing complex (GO:0032991)regulation of DNA-templated transcription (GO:0006355)regulation of transcription by RNA polymerase II (GO:0006357)regulation of transcription by RNA polymerase II (GO:0006357)regulation of transcription by RNA polymerase II (GO:0006357)regulation of translation (GO:0006417)response to dexamethasone (GO:0071548)response to fatty acid (GO:0070542)response to fatty acid (GO:0070542)response to nutrient levels (GO:0031667)response to starvation (GO:0042594)response to starvation (GO:0042594)response to starvation (GO:0042594)response to water-immersion restraint stress (GO:1990785)response to xenobiotic stimulus (GO:0009410)sequence-specific DNA binding (GO:0043565)sequence-specific DNA binding (GO:0043565)sequence-specific double-stranded DNA binding (GO:1990837)transcription cis-regulatory region binding (GO:0000976)transcription cis-regulatory region binding (GO:0000976)transcription coregulator binding (GO:0001221)tumor necrosis factor-mediated signaling pathway (GO:0033209)
Expression (TPM)
FOXO3 — as a Regulated Gene

TFs regulating FOXO3 0 TFs

Transcription factors with Perturb-seq knockdown data for FOXO3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FOXO3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to FOXO3

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FOXO3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr6:108,260,173–108,261,671 299.7 kb Distal (>10kb) Multiome 970
chr6:108,294,541–108,295,678 266.0 kb Distal (>10kb) Multiome 880
chr6:108,557,184–108,561,557 74 bp At TSS Multiome 1119
chr6:108,561,776–108,563,199 1.9 kb Proximal (<10kb) Multiome 782
chr6:108,781,208–108,782,610 220.6 kb Distal (>10kb) Multiome HiCAR 561
chr6:108,838,106–108,838,816 277.5 kb Distal (>10kb) Multiome HiCAR 212
chr6:108,847,848–108,848,955 287.5 kb Distal (>10kb) Multiome HiCAR 734

Genome Browser

Genomic view of the FOXO3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr6:108,250,173 – 108,858,955
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq