This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
Transcription factors with Perturb-seq knockdown data for FN1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FN1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FN1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:215,274,506–215,275,238 | 161.1 kb | Distal (>10kb) Multiome | 197 | |
| chr2:215,311,644–215,312,869 | 124.0 kb | Distal (>10kb) Multiome | 1061 | |
| chr2:215,327,589–215,328,665 | 107.8 kb | Distal (>10kb) Multiome | 170 | |
| chr2:215,435,401–215,436,973 | 60 bp | At TSS Multiome | 942 | |
| chr2:215,546,034–215,547,080 | 110.6 kb | Distal (>10kb) Multiome | 83 | |
| chr2:215,619,042–215,619,983 | 183.2 kb | Distal (>10kb) Multiome HiCAR | 750 | |
| chr2:215,905,935–215,906,837 | 470.4 kb | Distal (>10kb) Multiome HiCAR | 76 |
Genomic view of the FN1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.