Transcription factors with Perturb-seq knockdown data for FAM89A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FAM89A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FAM89A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:230,753,346–230,754,251 | 286.4 kb | Distal (>10kb) Multiome | 356 | |
| chr1:230,867,716–230,869,411 | 171.6 kb | Distal (>10kb) Multiome | 758 | |
| chr1:230,882,687–230,883,179 | 157.3 kb | Distal (>10kb) Multiome | 18 | |
| chr1:230,978,084–230,979,476 | 61.3 kb | Distal (>10kb) Multiome | 931 | |
| chr1:231,037,684–231,038,550 | 2.1 kb | Proximal (<10kb) Multiome | 274 | |
| chr1:231,039,156–231,041,267 | 36 bp | At TSS Multiome | 661 | |
| chr1:231,065,566–231,066,080 | 25.6 kb | Distal (>10kb) Multiome | 199 | |
| chr1:231,161,989–231,163,833 | 122.2 kb | Distal (>10kb) Multiome | 768 | |
| chr1:231,211,148–231,211,998 | 171.3 kb | Distal (>10kb) Multiome | 631 | |
| chr1:231,240,746–231,241,633 | 201.0 kb | Distal (>10kb) Multiome | 834 | |
| chr1:231,337,024–231,338,750 | 297.8 kb | Distal (>10kb) Multiome | 881 |
Genomic view of the FAM89A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.