Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in cellular response to oxidative stress; negative regulation of transcription by RNA polymerase II; and positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]
Modules significantly affected by knockdown. ↑ Up = module upregulated upon KD; ↓ Down = module downregulated upon KD.
| Cluster | Dir | NES | padj | Bind | OR | padj (bind) |
|---|
| Module | Dir | NES | #gRNA | padj | Bind | OR | padj (bind) |
|---|
| Submodule | Module | Dir | NES | #gRNA | Bind | OR | padj (bind) |
|---|
Genes likely regulated by ETV5 through linked binding evidence in open chromatin. The chart ranks TF-linked genes by their mean Perturb-seq response to ETV5 knockdown, with negative coefficients indicating downregulation and positive coefficients indicating upregulation upon knockdown.
Open chromatin elements (ATAC-seq) where ETV5 has ChIP-seq or motif footprint binding evidence and which are linked to at least one target gene region.
| Element | Size | Linked genes |
|---|
Transcription factors with Perturb-seq knockdown data for ETV5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ETV5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ETV5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:185,786,185–185,786,786 | 293.4 kb | Distal (>10kb) Multiome | 166 | |
| chr3:185,824,058–185,826,626 | 254.6 kb | Distal (>10kb) Multiome | 789 | |
| chr3:185,937,142–185,938,541 | 141.9 kb | Distal (>10kb) Multiome | 1100 | |
| chr3:185,943,380–185,943,981 | 136.3 kb | Distal (>10kb) Multiome | 545 | |
| chr3:185,983,585–185,984,068 | 96.1 kb | Distal (>10kb) Multiome | 356 | |
| chr3:186,079,815–186,080,525 | 185 bp | At TSS Multiome | 173 | |
| chr3:186,097,348–186,098,508 | 18.0 kb | Distal (>10kb) Multiome | 650 | |
| chr3:186,107,642–186,108,000 | 1.1 kb | Proximal (<10kb) | 478 | |
| chr3:186,108,150–186,110,388 | 29.3 kb | Distal (>10kb) Multiome | 901 | |
| chr3:186,193,414–186,194,695 | 114.1 kb | Distal (>10kb) Multiome | 264 | |
| chr3:186,241,891–186,242,871 | 162.7 kb | Distal (>10kb) Multiome | 584 | |
| chr3:186,259,091–186,259,922 | 179.8 kb | Distal (>10kb) Multiome | 406 | |
| chr3:186,361,726–186,362,566 | 282.3 kb | Distal (>10kb) Multiome | 278 | |
| chr3:186,382,314–186,383,518 | 302.8 kb | Distal (>10kb) Multiome | 146 |
Genomic view of the ETV5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.