Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for EPHB1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EPHB1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EPHB1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:134,650,423–134,651,401 | 144.3 kb | Distal (>10kb) Multiome | 242 | |
| chr3:134,794,772–134,796,898 | 59 bp | At TSS Multiome | 524 | |
| chr3:134,797,433–134,797,779 | 2.2 kb | Proximal (<10kb) | 56 | |
| chr3:134,937,414–134,938,571 | 142.7 kb | Distal (>10kb) Multiome | 132 | |
| chr3:135,067,209–135,067,929 | 272.3 kb | Distal (>10kb) Multiome | 42 | |
| chr3:135,503,661–135,504,167 | 708.7 kb | Distal (>10kb) Multiome HiCAR | 178 |
Genomic view of the EPHB1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.