Transcription factors with Perturb-seq knockdown data for ENSG00000227227. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ENSG00000227227 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ENSG00000227227, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:186,485,510–186,487,147 | 209.0 kb | Distal (>10kb) Multiome | 1078 | |
| chr2:186,589,400–186,591,227 | 105.2 kb | Distal (>10kb) Multiome | 1083 | |
| chr2:186,693,562–186,694,912 | 1.2 kb | Proximal (<10kb) Multiome | 615 | |
| chr2:186,695,033–186,695,340 | at TSS | At TSS | 49 | |
| chr2:186,734,517–186,735,706 | 40.1 kb | Distal (>10kb) Multiome | 224 | |
| chr2:186,848,474–186,849,953 | 153.9 kb | Distal (>10kb) Multiome | 410 |
Genomic view of the ENSG00000227227 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.