The protein encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ENSA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ENSA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ENSA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:150,363,368–150,364,980 | 265.6 kb | Distal (>10kb) Multiome | 971 | |
| chr1:150,487,154–150,487,710 | 142.2 kb | Distal (>10kb) Multiome | 929 | |
| chr1:150,515,085–150,516,227 | 113.9 kb | Distal (>10kb) Multiome | 781 | |
| chr1:150,548,849–150,549,823 | 80.3 kb | Distal (>10kb) Multiome | 620 | |
| chr1:150,560,828–150,562,623 | 68.2 kb | Distal (>10kb) Multiome | 897 | |
| chr1:150,578,554–150,580,300 | 50.0 kb | Distal (>10kb) Multiome | 990 | |
| chr1:150,627,434–150,628,113 | 1.0 kb | Proximal (<10kb) | 52 | |
| chr1:150,629,012–150,630,122 | 86 bp | At TSS Multiome | 875 | |
| chr1:150,634,804–150,635,486 | 5.6 kb | Proximal (<10kb) Multiome | 113 | |
| chr1:150,641,008–150,641,820 | 12.0 kb | Distal (>10kb) Multiome | 66 | |
| chr1:150,696,984–150,697,716 | 67.7 kb | Distal (>10kb) Multiome | 619 | |
| chr1:150,875,863–150,877,222 | 247.1 kb | Distal (>10kb) Multiome | 912 | |
| chr1:150,925,543–150,926,754 | 296.8 kb | Distal (>10kb) Multiome | 813 |
Genomic view of the ENSA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.