Predicted to enable phosphopyruvate hydratase activity. Predicted to be involved in glycolytic process. Predicted to act upstream of or within cilium organization and flagellated sperm motility. Predicted to be located in sperm principal piece. Predicted to be part of phosphopyruvate hydratase complex. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for ENO4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ENO4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ENO4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:116,608,834–116,610,180 | 239.7 kb | Distal (>10kb) Multiome | 307 | |
| chr10:116,627,107–116,628,019 | 221.9 kb | Distal (>10kb) Multiome | 282 | |
| chr10:116,629,218–116,630,194 | 219.9 kb | Distal (>10kb) Multiome | 56 | |
| chr10:116,669,744–116,670,994 | 179.3 kb | Distal (>10kb) Multiome | 324 | |
| chr10:116,741,030–116,743,588 | 106.8 kb | Distal (>10kb) Multiome | 566 | |
| chr10:116,833,883–116,834,686 | 15.3 kb | Distal (>10kb) Multiome | 209 | |
| chr10:116,836,058–116,836,660 | 13.2 kb | Distal (>10kb) Multiome | 190 | |
| chr10:116,848,918–116,850,542 | 100 bp | At TSS Multiome | 543 | |
| chr10:117,004,546–117,006,106 | 155.8 kb | Distal (>10kb) Multiome | 880 | |
| chr10:117,134,080–117,135,217 | 285.1 kb | Distal (>10kb) Multiome | 216 | |
| chr10:117,136,768–117,138,029 | 287.7 kb | Distal (>10kb) Multiome | 337 | |
| chr10:117,138,997–117,141,025 | 289.9 kb | Distal (>10kb) Multiome | 182 | |
| chr10:117,159,202–117,159,932 | 310.1 kb | Distal (>10kb) Multiome HiCAR | 133 | |
| chr10:117,162,523–117,164,008 | 313.7 kb | Distal (>10kb) Multiome HiCAR | 315 | |
| chr10:117,165,627–117,166,409 | 316.5 kb | Distal (>10kb) Multiome HiCAR | 272 | |
| chr10:117,167,701–117,168,673 | 318.8 kb | Distal (>10kb) Multiome HiCAR | 441 |
Genomic view of the ENO4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.