This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
Transcription factors with Perturb-seq knockdown data for ENO1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ENO1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ENO1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:8,702,848–8,703,691 | 175.3 kb | Distal (>10kb) Multiome | 839 | |
| chr1:8,817,213–8,818,248 | 60.9 kb | Distal (>10kb) Multiome | 791 | |
| chr1:8,848,753–8,849,552 | 29.6 kb | Distal (>10kb) Multiome | 603 | |
| chr1:8,855,525–8,856,275 | 22.9 kb | Distal (>10kb) Multiome | 37 | |
| chr1:8,873,547–8,874,366 | 4.7 kb | Proximal (<10kb) Multiome | 473 | |
| chr1:8,876,914–8,877,146 | 1.5 kb | Proximal (<10kb) | 169 | |
| chr1:8,877,947–8,879,611 | 334 bp | At TSS Multiome | 1144 | |
| chr1:8,883,593–8,884,123 | 4.9 kb | Proximal (<10kb) | 141 | |
| chr1:9,128,169–9,129,971 | 250.7 kb | Distal (>10kb) Multiome | 749 |
Genomic view of the ENO1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.