Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for EN1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EN1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EN1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:118,841,828–118,842,164 | 5.5 kb | Proximal (<10kb) | 80 | |
| chr2:118,842,787–118,843,412 | 4.2 kb | Proximal (<10kb) | 81 | |
| chr2:118,845,167–118,845,388 | 2.3 kb | Proximal (<10kb) | 103 | |
| chr2:118,845,738–118,847,412 | 235 bp | At TSS | 200 | |
| chr2:118,847,603–118,848,764 | at TSS | At TSS | 327 | |
| chr2:118,854,425–118,855,264 | 6.8 kb | Proximal (<10kb) | 70 | |
| chr2:118,855,419–118,856,205 | 7.8 kb | Proximal (<10kb) | 527 |
Genomic view of the EN1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.