EIF5

eukaryotic translation initiation factor 5

Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010]

Member of: DE-1 DE-1.7 Developmental clusters: GC4

Biological processes 17 terms

GDP-dissociation inhibitor activity (GO:0005092)RNA binding (GO:0003723)cadherin binding (GO:0045296)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)eukaryotic initiation factor eIF2 binding (GO:0071074)formation of cytoplasmic translation initiation complex (GO:0001732)nucleus (GO:0005634)protein binding (GO:0005515)regulation of translational initiation (GO:0006446)ribosome assembly (GO:0042255)synapse (GO:0045202)translation initiation factor activity (GO:0003743)translation initiation factor activity (GO:0003743)translational initiation (GO:0006413)

Expression (TPM)

TFs regulating EIF5 0 TFs

Transcription factors with Perturb-seq knockdown data for EIF5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EIF5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to EIF5

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EIF5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr14:103,056,337–103,058,497	276.6 kb	Distal (>10kb) Multiome	644
chr14:103,075,052–103,076,162	258.5 kb	Distal (>10kb) Multiome HiCAR	656
chr14:103,091,357–103,091,855	242.6 kb	Distal (>10kb) Multiome	384
chr14:103,104,655–103,105,370	229.1 kb	Distal (>10kb) Multiome	618
chr14:103,122,605–103,123,921	211.2 kb	Distal (>10kb) Multiome	378
chr14:103,126,688–103,127,597	207.1 kb	Distal (>10kb) Multiome	313
chr14:103,188,912–103,189,569	145.0 kb	Distal (>10kb) Multiome	138
chr14:103,207,129–103,207,830	126.8 kb	Distal (>10kb) Multiome	200
chr14:103,333,053–103,335,386	135 bp	At TSS Multiome	1004
chr14:103,384,755–103,386,021	51.1 kb	Distal (>10kb) Multiome	863
chr14:103,456,058–103,456,564	122.1 kb	Distal (>10kb) Multiome	49
chr14:103,518,427–103,519,043	184.6 kb	Distal (>10kb) Multiome	404
chr14:103,520,443–103,523,552	188.6 kb	Distal (>10kb) Multiome	756
chr14:103,528,671–103,529,619	194.9 kb	Distal (>10kb) Multiome	726
chr14:103,561,982–103,563,356	228.6 kb	Distal (>10kb) Multiome	781
chr14:103,628,110–103,630,126	294.9 kb	Distal (>10kb) Multiome	909

Genome Browser

Genomic view of the EIF5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.