The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for E2F5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = E2F5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of E2F5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:84,900,401–84,902,474 | 276.6 kb | Distal (>10kb) Multiome | 293 | |
| chr8:85,106,583–85,108,304 | 70.3 kb | Distal (>10kb) Multiome | 759 | |
| chr8:85,171,619–85,172,328 | 4.8 kb | Proximal (<10kb) | 160 | |
| chr8:85,176,569–85,178,114 | 332 bp | At TSS Multiome | 922 | |
| chr8:85,178,635–85,178,855 | 1.5 kb | Proximal (<10kb) | 74 | |
| chr8:85,219,878–85,221,874 | 42.9 kb | Distal (>10kb) Multiome | 895 | |
| chr8:85,244,896–85,246,268 | 68.1 kb | Distal (>10kb) Multiome | 796 | |
| chr8:85,463,010–85,465,876 | 286.2 kb | Distal (>10kb) Multiome | 649 | |
| chr8:86,068,911–86,069,915 | 891.8 kb | Distal (>10kb) Multiome HiCAR | 240 |
Genomic view of the E2F5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.