Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for DRAP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DRAP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DRAP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:65,637,768–65,638,920 | 281.3 kb | Distal (>10kb) Multiome | 740 | |
| chr11:65,641,918–65,642,633 | 277.3 kb | Distal (>10kb) Multiome | 155 | |
| chr11:65,646,814–65,647,818 | 272.1 kb | Distal (>10kb) Multiome | 270 | |
| chr11:65,652,106–65,653,317 | 266.7 kb | Distal (>10kb) Multiome | 901 | |
| chr11:65,662,517–65,663,930 | 256.4 kb | Distal (>10kb) Multiome | 839 | |
| chr11:65,711,437–65,712,942 | 207.1 kb | Distal (>10kb) Multiome | 1143 | |
| chr11:65,720,215–65,720,955 | 198.7 kb | Distal (>10kb) Multiome | 821 | |
| chr11:65,779,808–65,781,988 | 139.4 kb | Distal (>10kb) Multiome | 764 | |
| chr11:65,785,898–65,789,101 | 131.0 kb | Distal (>10kb) Multiome | 478 | |
| chr11:65,817,968–65,818,502 | 101.2 kb | Distal (>10kb) Multiome | 694 | |
| chr11:65,833,325–65,834,189 | 85.6 kb | Distal (>10kb) Multiome | 396 | |
| chr11:65,856,804–65,858,748 | 62.4 kb | Distal (>10kb) Multiome | 972 | |
| chr11:65,859,034–65,861,240 | 58.8 kb | Distal (>10kb) Multiome | 1012 | |
| chr11:65,871,920–65,874,072 | 45.7 kb | Distal (>10kb) Multiome | 741 | |
| chr11:65,887,987–65,889,162 | 30.9 kb | Distal (>10kb) Multiome | 824 | |
| chr11:65,890,253–65,891,457 | 28.8 kb | Distal (>10kb) Multiome | 895 | |
| chr11:65,899,867–65,900,779 | 18.9 kb | Distal (>10kb) Multiome | 740 | |
| chr11:65,918,507–65,920,266 | 180 bp | At TSS Multiome | 992 | |
| chr11:65,961,271–65,962,172 | 42.2 kb | Distal (>10kb) Multiome | 834 | |
| chr11:66,001,882–66,002,962 | 83.0 kb | Distal (>10kb) Multiome | 1022 | |
| chr11:66,007,227–66,007,703 | 88.1 kb | Distal (>10kb) Multiome | 100 | |
| chr11:66,022,341–66,022,903 | 103.2 kb | Distal (>10kb) Multiome | 466 | |
| chr11:66,045,170–66,045,637 | 125.9 kb | Distal (>10kb) Multiome | 394 | |
| chr11:66,048,912–66,049,460 | 129.7 kb | Distal (>10kb) Multiome | 83 | |
| chr11:66,051,716–66,052,760 | 132.8 kb | Distal (>10kb) Multiome | 901 | |
| chr11:66,069,690–66,071,102 | 150.7 kb | Distal (>10kb) Multiome | 622 |
Genomic view of the DRAP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.