This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009]
Transcription factors with Perturb-seq knockdown data for DOHH. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DOHH upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DOHH, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:3,224,482–3,224,897 | 276.0 kb | Distal (>10kb) Multiome | 253 | |
| chr19:3,275,036–3,277,129 | 224.4 kb | Distal (>10kb) Multiome | 737 | |
| chr19:3,284,276–3,286,396 | 215.1 kb | Distal (>10kb) Multiome | 555 | |
| chr19:3,360,114–3,360,563 | 140.4 kb | Distal (>10kb) Multiome | 430 | |
| chr19:3,434,837–3,435,636 | 65.5 kb | Distal (>10kb) Multiome | 777 | |
| chr19:3,441,686–3,442,206 | 58.7 kb | Distal (>10kb) Multiome | 86 | |
| chr19:3,444,085–3,444,710 | 56.2 kb | Distal (>10kb) Multiome | 150 | |
| chr19:3,494,144–3,494,348 | 6.3 kb | Proximal (<10kb) | 65 | |
| chr19:3,500,403–3,501,074 | 129 bp | At TSS Multiome | 886 | |
| chr19:3,505,575–3,507,061 | 5.4 kb | Proximal (<10kb) Multiome | 838 | |
| chr19:3,507,612–3,508,152 | 6.9 kb | Proximal (<10kb) | 266 | |
| chr19:3,557,019–3,558,238 | 57.0 kb | Distal (>10kb) Multiome | 811 | |
| chr19:3,572,147–3,574,664 | 72.0 kb | Distal (>10kb) Multiome | 737 | |
| chr19:3,576,363–3,578,543 | 77.6 kb | Distal (>10kb) Multiome | 346 | |
| chr19:3,606,342–3,607,177 | 106.2 kb | Distal (>10kb) Multiome | 377 | |
| chr19:3,626,518–3,627,304 | 126.4 kb | Distal (>10kb) Multiome | 833 | |
| chr19:3,699,968–3,700,899 | 199.8 kb | Distal (>10kb) Multiome | 489 | |
| chr19:3,721,462–3,721,997 | 221.0 kb | Distal (>10kb) Multiome | 760 | |
| chr19:3,722,652–3,723,093 | 222.3 kb | Distal (>10kb) Multiome | 106 | |
| chr19:3,761,103–3,762,150 | 260.9 kb | Distal (>10kb) Multiome | 839 | |
| chr19:3,762,300–3,763,344 | 262.2 kb | Distal (>10kb) Multiome | 817 | |
| chr19:3,786,012–3,786,456 | 285.5 kb | Distal (>10kb) Multiome | 237 |
Genomic view of the DOHH locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.