DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for DNAJC6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DNAJC6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DNAJC6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:64,965,270–64,967,021 | 287.9 kb | Distal (>10kb) Multiome | 764 | |
| chr1:65,002,269–65,003,167 | 251.7 kb | Distal (>10kb) Multiome | 584 | |
| chr1:65,065,952–65,068,343 | 187.9 kb | Distal (>10kb) Multiome | 865 | |
| chr1:65,147,108–65,150,062 | 106.7 kb | Distal (>10kb) Multiome | 912 | |
| chr1:65,253,859–65,255,712 | 34 bp | At TSS Multiome | 756 | |
| chr1:65,256,994–65,257,571 | 2.5 kb | Proximal (<10kb) | 61 | |
| chr1:65,264,205–65,264,999 | 9.7 kb | Proximal (<10kb) | 289 | |
| chr1:65,265,365–65,266,424 | 11.4 kb | Distal (>10kb) Multiome | 602 | |
| chr1:65,308,757–65,310,566 | 55.5 kb | Distal (>10kb) Multiome | 950 | |
| chr1:65,419,304–65,421,455 | 165.9 kb | Distal (>10kb) Multiome | 961 | |
| chr1:65,467,350–65,468,066 | 213.2 kb | Distal (>10kb) Multiome | 382 | |
| chr1:65,687,566–65,688,519 | 433.5 kb | Distal (>10kb) Multiome HiCAR | 622 | |
| chr1:66,532,636–66,534,688 | 1279.5 kb | Distal (>10kb) Multiome HiCAR | 428 |
Genomic view of the DNAJC6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.