This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Modules significantly affected by knockdown. ↑ Up = module upregulated upon KD; ↓ Down = module downregulated upon KD.
| Module | Dir | NES | #gRNA | padj | Bind | OR | padj (bind) |
|---|
| Submodule | Module | Dir | NES | #gRNA | Bind | OR | padj (bind) |
|---|
Genes likely regulated by DNAJC2 through linked binding evidence in open chromatin. The chart ranks TF-linked genes by their mean Perturb-seq response to DNAJC2 knockdown, with negative coefficients indicating downregulation and positive coefficients indicating upregulation upon knockdown.
Open chromatin elements (ATAC-seq) where DNAJC2 has ChIP-seq or motif footprint binding evidence and which are linked to at least one target gene region.
| Element | Size | Linked genes |
|---|
Transcription factors with Perturb-seq knockdown data for DNAJC2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DNAJC2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DNAJC2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:103,074,047–103,075,916 | 269.8 kb | Distal (>10kb) Multiome | 860 | |
| chr7:103,148,730–103,149,910 | 195.5 kb | Distal (>10kb) Multiome | 1002 | |
| chr7:103,279,769–103,280,793 | 64.4 kb | Distal (>10kb) Multiome | 291 | |
| chr7:103,297,040–103,297,853 | 47.4 kb | Distal (>10kb) Multiome | 880 | |
| chr7:103,344,148–103,345,138 | 72 bp | At TSS Multiome | 994 | |
| chr7:103,347,517–103,348,162 | 2.9 kb | Proximal (<10kb) Multiome | 795 | |
| chr7:103,445,161–103,446,559 | 101.2 kb | Distal (>10kb) Multiome | 424 | |
| chr7:103,597,491–103,598,216 | 253.0 kb | Distal (>10kb) Multiome | 73 | |
| chr7:104,944,876–104,945,547 | 1600.4 kb | Distal (>10kb) Multiome HiCAR | 743 |
Genomic view of the DNAJC2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.