This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Transcription factors with Perturb-seq knockdown data for DNAJB1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DNAJB1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DNAJB1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:12,656,871–12,657,566 | 1872.1 kb | Distal (>10kb) Multiome HiCAR | 254 | |
| chr19:12,669,351–12,669,939 | 1859.7 kb | Distal (>10kb) Multiome HiCAR | 777 | |
| chr19:14,247,378–14,248,008 | 281.7 kb | Distal (>10kb) Multiome | 109 | |
| chr19:14,248,166–14,249,635 | 280.5 kb | Distal (>10kb) Multiome | 230 | |
| chr19:14,264,813–14,266,130 | 263.8 kb | Distal (>10kb) Multiome | 223 | |
| chr19:14,294,831–14,295,420 | 234.1 kb | Distal (>10kb) Multiome | 60 | |
| chr19:14,363,590–14,364,020 | 165.6 kb | Distal (>10kb) Multiome | 402 | |
| chr19:14,405,442–14,406,893 | 123.1 kb | Distal (>10kb) Multiome | 662 | |
| chr19:14,418,847–14,419,795 | 109.8 kb | Distal (>10kb) Multiome | 817 | |
| chr19:14,432,848–14,433,780 | 95.9 kb | Distal (>10kb) Multiome | 524 | |
| chr19:14,450,232–14,451,000 | 78.6 kb | Distal (>10kb) Multiome | 271 | |
| chr19:14,472,177–14,472,986 | 56.7 kb | Distal (>10kb) Multiome | 566 | |
| chr19:14,495,705–14,496,690 | 33.0 kb | Distal (>10kb) Multiome | 813 | |
| chr19:14,517,523–14,519,160 | 10.7 kb | Distal (>10kb) Multiome | 952 | |
| chr19:14,528,857–14,530,279 | 191 bp | At TSS Multiome | 842 | |
| chr19:14,536,700–14,537,683 | 7.9 kb | Proximal (<10kb) Multiome | 329 | |
| chr19:14,556,545–14,557,088 | 27.5 kb | Distal (>10kb) Multiome | 509 | |
| chr19:14,564,060–14,565,155 | 35.2 kb | Distal (>10kb) Multiome | 226 | |
| chr19:14,571,772–14,572,330 | 42.8 kb | Distal (>10kb) Multiome | 896 | |
| chr19:14,689,407–14,690,497 | 160.7 kb | Distal (>10kb) Multiome | 624 | |
| chr19:16,324,466–16,326,401 | 1796.8 kb | Distal (>10kb) Multiome HiCAR | 477 |
Genomic view of the DNAJB1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.