DNA2
DNA replication helicase/nuclease 2 | KIAA0083, DNA2L

This gene encodes a member of the DNA2/NAM7 helicase family. The encoded protein is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability. Mutations in this gene are associated with autosomal dominant progressive external ophthalmoplegia-6 (PEOA6) and Seckel syndrome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]

Member of: DE-6 DE-6.1 Developmental clusters: GC4
Biological processes 48 terms
5'-3' DNA helicase activity (GO:0043139)5'-flap endonuclease activity (GO:0017108)5'-flap endonuclease activity (GO:0017108)5'-flap endonuclease activity (GO:0017108)5'-flap endonuclease activity (GO:0017108)ATP hydrolysis activity (GO:0016887)ATP hydrolysis activity (GO:0016887)ATP hydrolysis activity (GO:0016887)DNA binding (GO:0003677)DNA double-strand break processing (GO:0000729)DNA geometric change (GO:0032392)DNA helicase activity (GO:0003678)DNA helicase activity (GO:0003678)DNA replication (GO:0006260)DNA replication checkpoint signaling (GO:0000076)DNA replication, Okazaki fragment processing (GO:0033567)DNA replication, removal of RNA primer (GO:0043137)RNA binding (GO:0003723)base-excision repair (GO:0006284)base-excision repair (GO:0006284)chromosome, telomeric region (GO:0000781)chromosome, telomeric region (GO:0000781)cytoplasm (GO:0005737)deoxyribonuclease (pyrimidine dimer) activity (GO:0033892)helicase activity (GO:0004386)helicase activity (GO:0004386)mitochondrial DNA repair (GO:0043504)mitochondrial DNA replication (GO:0006264)mitochondrial nucleoid (GO:0042645)mitochondrion (GO:0005739)mitochondrion (GO:0005739)mitochondrion (GO:0005739)mitotic telomere maintenance via semi-conservative replication (GO:1902990)mitotic telomere maintenance via semi-conservative replication (GO:1902990)nuclease activity (GO:0004518)nuclease activity (GO:0004518)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of DNA replication (GO:0045740)protein binding (GO:0005515)replication fork reversal (GO:0071932)single-stranded DNA helicase activity (GO:0017116)t-circle formation (GO:0090656)telomere maintenance (GO:0000723)telomere maintenance (GO:0000723)telomere maintenance via semi-conservative replication (GO:0032201)telomere maintenance via semi-conservative replication (GO:0032201)
Expression (TPM)
DNA2 — as a Regulated Gene

TFs regulating DNA2 0 TFs

Transcription factors with Perturb-seq knockdown data for DNA2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DNA2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to DNA2

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DNA2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr10:68,208,472–68,208,961 263.3 kb Distal (>10kb) Multiome 254
chr10:68,231,028–68,232,253 240.5 kb Distal (>10kb) Multiome 654
chr10:68,271,165–68,272,025 200.4 kb Distal (>10kb) Multiome 177
chr10:68,331,343–68,333,064 139.0 kb Distal (>10kb) Multiome 1174
chr10:68,406,245–68,407,662 64.7 kb Distal (>10kb) Multiome 898
chr10:68,471,473–68,472,528 41 bp At TSS Multiome 831
chr10:68,527,011–68,528,012 55.6 kb Distal (>10kb) Multiome 912
chr10:68,559,963–68,561,065 88.4 kb Distal (>10kb) Multiome 777
chr10:68,600,036–68,600,760 128.5 kb Distal (>10kb) Multiome 755
chr10:68,720,659–68,721,908 249.2 kb Distal (>10kb) Multiome 939

Genome Browser

Genomic view of the DNA2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr10:68,198,472 – 68,731,908
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq