The pattern of cellular proliferation and differentiation that leads to normal development of embryonic structures often depends upon the localized production of secreted protein signals. Cells surrounding the source of a particular signal respond in a graded manner according to the effective concentration of the signal, and this response produces the pattern of cell types constituting the mature structure. A novel segment-polarity gene known as dispatched has been identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. This gene is one of two human homologs of Drosophila dispatched and, based on sequence identity to its mouse counterpart, the encoded protein may play an essential role in Hh patterning activities in the early embryo. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for DISP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DISP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DISP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:222,589,519–222,590,254 | 225.2 kb | Distal (>10kb) Multiome | 689 | |
| chr1:222,617,261–222,619,333 | 197.2 kb | Distal (>10kb) Multiome | 690 | |
| chr1:222,643,693–222,644,710 | 170.8 kb | Distal (>10kb) Multiome | 917 | |
| chr1:222,711,408–222,713,804 | 102.5 kb | Distal (>10kb) Multiome | 1037 | |
| chr1:222,814,519–222,815,531 | 6 bp | At TSS Multiome | 704 | |
| chr1:223,022,274–223,024,726 | 208.1 kb | Distal (>10kb) Multiome | 430 | |
| chr1:223,029,922–223,031,363 | 215.4 kb | Distal (>10kb) Multiome | 295 | |
| chr1:223,081,188–223,081,710 | 266.4 kb | Distal (>10kb) Multiome HiCAR | 712 | |
| chr1:223,129,163–223,129,910 | 314.5 kb | Distal (>10kb) Multiome HiCAR | 411 |
Genomic view of the DISP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.