Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
Transcription factors with Perturb-seq knockdown data for DHFR. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DHFR upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DHFR, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:80,407,421–80,408,673 | 246.5 kb | Distal (>10kb) Multiome | 954 | |
| chr5:80,487,503–80,488,829 | 166.4 kb | Distal (>10kb) Multiome HiCAR | 871 | |
| chr5:80,554,442–80,555,055 | 99.7 kb | Distal (>10kb) Multiome | 148 | |
| chr5:80,568,978–80,570,765 | 84.2 kb | Distal (>10kb) Multiome | 431 | |
| chr5:80,645,035–80,645,238 | 9.3 kb | Proximal (<10kb) | 24 | |
| chr5:80,654,076–80,655,416 | 327 bp | At TSS Multiome | 820 |
Genomic view of the DHFR locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.