This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this gene encoding distinct isoforms are identified. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for DENND2B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DENND2B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DENND2B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:8,593,749–8,594,754 | 316.8 kb | Distal (>10kb) Multiome | 587 | |
| chr11:8,671,011–8,671,925 | 239.6 kb | Distal (>10kb) Multiome | 223 | |
| chr11:8,682,170–8,683,291 | 228.3 kb | Distal (>10kb) Multiome HiCAR | 956 | |
| chr11:8,687,635–8,689,327 | 221.9 kb | Distal (>10kb) Multiome HiCAR | 1023 | |
| chr11:8,700,857–8,701,532 | 209.7 kb | Distal (>10kb) Multiome | 216 | |
| chr11:8,713,673–8,714,258 | 197.0 kb | Distal (>10kb) Multiome HiCAR | 102 | |
| chr11:8,800,375–8,801,511 | 109.9 kb | Distal (>10kb) Multiome | 167 | |
| chr11:8,810,380–8,811,275 | 100.2 kb | Distal (>10kb) Multiome | 360 | |
| chr11:8,813,629–8,814,688 | 97.1 kb | Distal (>10kb) Multiome | 380 | |
| chr11:8,870,357–8,871,640 | 40.0 kb | Distal (>10kb) Multiome | 506 | |
| chr11:8,909,732–8,910,606 | 344 bp | At TSS | 128 | |
| chr11:8,910,714–8,911,839 | 270 bp | At TSS Multiome | 722 | |
| chr11:8,963,907–8,965,344 | 53.5 kb | Distal (>10kb) Multiome | 912 | |
| chr11:9,003,516–9,004,824 | 93.1 kb | Distal (>10kb) Multiome | 534 | |
| chr11:9,090,882–9,091,926 | 180.5 kb | Distal (>10kb) Multiome | 196 |
Genomic view of the DENND2B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.