This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2017]
Transcription factors with Perturb-seq knockdown data for DDX5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DDX5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DDX5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:64,262,431–64,263,974 | 242.2 kb | Distal (>10kb) Multiome | 914 | |
| chr17:64,470,609–64,471,086 | 34.7 kb | Distal (>10kb) Multiome | 39 | |
| chr17:64,496,498–64,497,782 | 8.4 kb | Proximal (<10kb) Multiome | 1074 | |
| chr17:64,504,990–64,507,698 | 238 bp | At TSS Multiome | 1232 | |
| chr17:64,661,214–64,662,931 | 156.7 kb | Distal (>10kb) Multiome | 700 | |
| chr17:64,773,965–64,774,847 | 268.8 kb | Distal (>10kb) Multiome | 481 | |
| chr17:64,776,631–64,777,384 | 271.5 kb | Distal (>10kb) Multiome | 616 | |
| chr17:64,778,215–64,779,097 | 273.1 kb | Distal (>10kb) Multiome | 185 | |
| chr17:64,779,868–64,781,331 | 274.8 kb | Distal (>10kb) Multiome | 287 |
Genomic view of the DDX5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.