This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]
Transcription factors with Perturb-seq knockdown data for DCLK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DCLK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DCLK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr13:35,621,090–35,622,512 | 509.3 kb | Distal (>10kb) Multiome | 138 | |
| chr13:35,855,376–35,856,583 | 275.6 kb | Distal (>10kb) Multiome | 382 | |
| chr13:35,857,690–35,858,256 | 2.0 kb | Proximal (<10kb) | 86 | |
| chr13:36,130,286–36,131,909 | 186 bp | At TSS Multiome | 348 | |
| chr13:36,297,356–36,298,329 | 166.5 kb | Distal (>10kb) Multiome | 597 | |
| chr13:36,298,442–36,299,112 | 167.5 kb | Distal (>10kb) Multiome | 192 | |
| chr13:36,324,257–36,325,131 | 193.2 kb | Distal (>10kb) Multiome | 143 | |
| chr13:36,345,510–36,347,162 | 215.3 kb | Distal (>10kb) Multiome | 776 |
Genomic view of the DCLK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.