This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for DBI. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DBI upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DBI, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:119,155,495–119,157,277 | 211.0 kb | Distal (>10kb) Multiome | 202 | |
| chr2:119,218,121–119,219,066 | 148.2 kb | Distal (>10kb) Multiome | 361 | |
| chr2:119,223,024–119,224,452 | 143.2 kb | Distal (>10kb) Multiome | 761 | |
| chr2:119,366,351–119,367,938 | 12 bp | At TSS Multiome | 994 | |
| chr2:119,431,298–119,432,803 | 64.8 kb | Distal (>10kb) Multiome | 903 | |
| chr2:119,476,117–119,476,795 | 109.5 kb | Distal (>10kb) Multiome | 482 | |
| chr2:119,523,786–119,524,663 | 157.4 kb | Distal (>10kb) Multiome | 187 | |
| chr2:119,537,405–119,538,283 | 170.9 kb | Distal (>10kb) Multiome | 122 | |
| chr2:119,544,074–119,545,082 | 177.5 kb | Distal (>10kb) Multiome | 610 |
Genomic view of the DBI locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.