In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for DAZAP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DAZAP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DAZAP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:1,112,029–1,112,884 | 295.4 kb | Distal (>10kb) Multiome | 294 | |
| chr19:1,113,014–1,114,139 | 294.1 kb | Distal (>10kb) Multiome | 690 | |
| chr19:1,122,934–1,123,720 | 284.5 kb | Distal (>10kb) Multiome | 231 | |
| chr19:1,173,441–1,174,438 | 233.4 kb | Distal (>10kb) Multiome | 682 | |
| chr19:1,194,370–1,194,985 | 213.0 kb | Distal (>10kb) Multiome | 531 | |
| chr19:1,205,175–1,206,374 | 202.1 kb | Distal (>10kb) Multiome | 746 | |
| chr19:1,237,080–1,238,434 | 169.6 kb | Distal (>10kb) Multiome | 430 | |
| chr19:1,239,513–1,242,539 | 165.9 kb | Distal (>10kb) Multiome | 889 | |
| chr19:1,244,253–1,244,857 | 163.1 kb | Distal (>10kb) Multiome | 590 | |
| chr19:1,247,981–1,252,501 | 156.6 kb | Distal (>10kb) Multiome | 1136 | |
| chr19:1,259,185–1,262,955 | 148.3 kb | Distal (>10kb) Multiome | 1070 | |
| chr19:1,265,280–1,270,235 | 138.4 kb | Distal (>10kb) Multiome | 1171 | |
| chr19:1,274,423–1,276,502 | 131.9 kb | Distal (>10kb) Multiome | 967 | |
| chr19:1,285,317–1,286,108 | 121.9 kb | Distal (>10kb) Multiome | 277 | |
| chr19:1,354,303–1,355,411 | 52.8 kb | Distal (>10kb) Multiome | 688 | |
| chr19:1,383,122–1,384,333 | 24.0 kb | Distal (>10kb) Multiome | 1228 | |
| chr19:1,401,101–1,401,839 | 6.1 kb | Proximal (<10kb) Multiome | 540 | |
| chr19:1,406,589–1,408,783 | 249 bp | At TSS Multiome | 911 | |
| chr19:1,415,008–1,415,721 | 7.8 kb | Proximal (<10kb) Multiome | 597 | |
| chr19:1,437,988–1,438,998 | 30.7 kb | Distal (>10kb) Multiome | 1076 | |
| chr19:1,444,785–1,447,383 | 37.6 kb | Distal (>10kb) Multiome | 797 | |
| chr19:1,455,191–1,456,421 | 48.1 kb | Distal (>10kb) Multiome | 491 | |
| chr19:1,468,941–1,469,903 | 61.6 kb | Distal (>10kb) Multiome | 242 | |
| chr19:1,470,088–1,471,597 | 63.1 kb | Distal (>10kb) Multiome | 544 | |
| chr19:1,478,772–1,479,848 | 71.7 kb | Distal (>10kb) Multiome | 857 | |
| chr19:1,489,566–1,491,829 | 83.2 kb | Distal (>10kb) Multiome | 836 | |
| chr19:1,507,230–1,508,699 | 100.5 kb | Distal (>10kb) Multiome | 596 | |
| chr19:1,512,960–1,513,686 | 105.5 kb | Distal (>10kb) Multiome | 541 | |
| chr19:1,523,798–1,524,624 | 116.4 kb | Distal (>10kb) Multiome | 263 | |
| chr19:1,566,277–1,568,916 | 159.1 kb | Distal (>10kb) Multiome | 748 | |
| chr19:1,592,085–1,593,444 | 185.1 kb | Distal (>10kb) Multiome | 883 | |
| chr19:1,604,859–1,606,501 | 198.0 kb | Distal (>10kb) Multiome | 902 | |
| chr19:1,651,519–1,653,514 | 245.3 kb | Distal (>10kb) Multiome | 909 |
Genomic view of the DAZAP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.