DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for DAD1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DAD1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DAD1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:22,377,714–22,378,505 | 211.0 kb | Distal (>10kb) Multiome | 131 | |
| chr14:22,556,665–22,557,670 | 32.0 kb | Distal (>10kb) Multiome | 771 | |
| chr14:22,560,678–22,561,385 | 28.2 kb | Distal (>10kb) Multiome | 502 | |
| chr14:22,570,258–22,571,116 | 18.7 kb | Distal (>10kb) Multiome | 460 | |
| chr14:22,588,929–22,590,086 | 230 bp | At TSS Multiome | 964 | |
| chr14:22,593,311–22,593,528 | 4.1 kb | Proximal (<10kb) | 14 | |
| chr14:22,597,878–22,599,136 | 9.1 kb | Proximal (<10kb) Multiome | 834 | |
| chr14:22,766,419–22,767,375 | 177.5 kb | Distal (>10kb) Multiome | 988 | |
| chr14:22,821,826–22,823,530 | 233.7 kb | Distal (>10kb) Multiome | 649 | |
| chr14:22,829,704–22,830,328 | 240.7 kb | Distal (>10kb) Multiome | 871 | |
| chr14:22,836,107–22,837,017 | 247.2 kb | Distal (>10kb) Multiome | 320 | |
| chr14:22,852,845–22,853,709 | 264.1 kb | Distal (>10kb) Multiome | 788 | |
| chr14:22,870,967–22,873,202 | 282.4 kb | Distal (>10kb) Multiome | 1167 | |
| chr14:22,882,594–22,883,886 | 294.0 kb | Distal (>10kb) Multiome | 289 | |
| chr14:22,886,371–22,887,824 | 298.0 kb | Distal (>10kb) Multiome | 639 |
Genomic view of the DAD1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.