Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2012]
Transcription factors with Perturb-seq knockdown data for DAAM1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DAAM1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DAAM1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:59,184,074–59,185,411 | 3.3 kb | Proximal (<10kb) | 145 | |
| chr14:59,187,813–59,190,715 | 1.2 kb | Proximal (<10kb) Multiome | 930 | |
| chr14:59,266,648–59,267,455 | 78.3 kb | Distal (>10kb) Multiome | 53 | |
| chr14:59,445,381–59,446,068 | 257.1 kb | Distal (>10kb) Multiome HiCAR | 78 | |
| chr14:59,463,731–59,465,599 | 276.4 kb | Distal (>10kb) Multiome | 584 | |
| chr14:59,483,421–59,485,055 | 295.7 kb | Distal (>10kb) Multiome | 1082 |
Genomic view of the DAAM1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.