Transcription factors with Perturb-seq knockdown data for CYP4A22-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CYP4A22-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CYP4A22-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:46,616,228–46,617,226 | 562.7 kb | Distal (>10kb) Multiome HiCAR | 517 | |
| chr1:47,023,511–47,024,146 | 155.6 kb | Distal (>10kb) Multiome | 258 | |
| chr1:47,175,406–47,175,819 | 3.5 kb | Proximal (<10kb) | 213 | |
| chr1:47,179,069–47,179,667 | 36 bp | At TSS Multiome | 660 | |
| chr1:47,181,080–47,181,435 | 1.8 kb | Proximal (<10kb) | 422 | |
| chr1:47,192,732–47,193,390 | 13.8 kb | Distal (>10kb) Multiome | 573 | |
| chr1:47,208,388–47,208,956 | 29.4 kb | Distal (>10kb) Multiome | 76 | |
| chr1:47,307,736–47,308,358 | 128.8 kb | Distal (>10kb) Multiome | 63 | |
| chr1:47,313,819–47,314,595 | 135.0 kb | Distal (>10kb) Multiome | 830 | |
| chr1:47,333,339–47,334,866 | 154.7 kb | Distal (>10kb) Multiome | 912 | |
| chr1:47,416,109–47,417,449 | 237.6 kb | Distal (>10kb) Multiome | 138 | |
| chr1:47,424,446–47,424,949 | 245.4 kb | Distal (>10kb) Multiome | 274 | |
| chr1:47,432,258–47,433,058 | 253.4 kb | Distal (>10kb) Multiome | 298 | |
| chr1:47,436,505–47,439,686 | 258.6 kb | Distal (>10kb) Multiome | 844 | |
| chr1:47,449,968–47,450,449 | 270.9 kb | Distal (>10kb) Multiome | 195 |
Genomic view of the CYP4A22-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.