This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]
Transcription factors with Perturb-seq knockdown data for CRKL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CRKL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CRKL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:20,702,218–20,703,674 | 214.4 kb | Distal (>10kb) Multiome | 438 | |
| chr22:20,857,959–20,859,512 | 58.7 kb | Distal (>10kb) Multiome | 901 | |
| chr22:20,885,370–20,886,088 | 31.6 kb | Distal (>10kb) Multiome | 749 | |
| chr22:20,916,779–20,918,119 | 182 bp | At TSS Multiome | 985 | |
| chr22:20,957,739–20,958,321 | 40.4 kb | Distal (>10kb) Multiome | 346 | |
| chr22:20,979,087–20,980,257 | 62.1 kb | Distal (>10kb) Multiome | 435 | |
| chr22:20,981,807–20,982,763 | 64.7 kb | Distal (>10kb) Multiome | 1020 | |
| chr22:21,001,335–21,002,797 | 84.6 kb | Distal (>10kb) Multiome | 1021 | |
| chr22:21,013,880–21,015,021 | 96.9 kb | Distal (>10kb) Multiome | 661 | |
| chr22:21,045,516–21,046,316 | 128.4 kb | Distal (>10kb) Multiome | 414 |
Genomic view of the CRKL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.