cAMP response element (CRE)-binding protein-like-2 (CREBL2) was identified in a search to find genes in a commonly deleted region on chromosome 12p13 flanked by ETV6 and CDKN1B genes, frequently associated with hematopoietic malignancies, as well as breast, non-small-cell lung and ovarian cancers. CREBL2 shares a 41% identity with CRE-binding protein (CREB) over a 48-base long region which encodes the bZip domain of CREB. The bZip domain consists of about 30 amino acids rich in basic residues involved in DNA binding, followed by a leucine zipper motif involved in protein dimerization. This suggests that CREBL2 encodes a protein with DNA binding capabilities. The occurance of CREBL2 deletion in malignancy suggests that CREBL2 may act as a tumor suppressor gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CREBL2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CREBL2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CREBL2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:12,349,790–12,350,566 | 261.6 kb | Distal (>10kb) Multiome | 671 | |
| chr12:12,356,841–12,357,808 | 254.8 kb | Distal (>10kb) Multiome | 912 | |
| chr12:12,561,525–12,563,261 | 49.1 kb | Distal (>10kb) Multiome | 768 | |
| chr12:12,611,502–12,612,657 | 47 bp | At TSS Multiome | 980 | |
| chr12:12,684,299–12,685,064 | 72.9 kb | Distal (>10kb) Multiome | 306 | |
| chr12:12,693,129–12,694,039 | 81.7 kb | Distal (>10kb) Multiome | 190 | |
| chr12:12,695,955–12,697,008 | 84.5 kb | Distal (>10kb) Multiome | 568 | |
| chr12:12,716,729–12,717,910 | 105.4 kb | Distal (>10kb) Multiome | 799 | |
| chr12:12,723,708–12,726,164 | 113.7 kb | Distal (>10kb) Multiome | 958 | |
| chr12:12,742,773–12,743,975 | 131.4 kb | Distal (>10kb) Multiome | 118 | |
| chr12:12,775,741–12,776,742 | 164.5 kb | Distal (>10kb) Multiome | 267 | |
| chr12:12,786,507–12,787,490 | 175.1 kb | Distal (>10kb) Multiome | 739 | |
| chr12:12,799,298–12,799,907 | 187.8 kb | Distal (>10kb) Multiome | 137 | |
| chr12:12,812,936–12,813,661 | 201.4 kb | Distal (>10kb) Multiome | 700 | |
| chr12:12,890,573–12,891,789 | 279.0 kb | Distal (>10kb) Multiome | 656 |
Genomic view of the CREBL2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.