Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CPSF4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CPSF4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CPSF4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:99,142,006–99,144,629 | 296.6 kb | Distal (>10kb) Multiome | 865 | |
| chr7:99,325,304–99,326,401 | 113.1 kb | Distal (>10kb) Multiome | 858 | |
| chr7:99,373,955–99,375,619 | 63.4 kb | Distal (>10kb) Multiome | 759 | |
| chr7:99,392,361–99,394,025 | 45.8 kb | Distal (>10kb) Multiome | 486 | |
| chr7:99,407,975–99,409,432 | 30.1 kb | Distal (>10kb) Multiome | 975 | |
| chr7:99,431,903–99,432,400 | 6.5 kb | Proximal (<10kb) | 30 | |
| chr7:99,438,371–99,439,668 | 125 bp | At TSS Multiome | 881 | |
| chr7:99,465,842–99,466,381 | 27.2 kb | Distal (>10kb) Multiome | 949 | |
| chr7:99,471,690–99,473,325 | 33.8 kb | Distal (>10kb) Multiome | 861 | |
| chr7:99,499,759–99,500,629 | 61.3 kb | Distal (>10kb) Multiome | 809 | |
| chr7:99,504,224–99,505,507 | 65.8 kb | Distal (>10kb) Multiome | 881 | |
| chr7:99,557,927–99,559,319 | 119.7 kb | Distal (>10kb) Multiome | 922 | |
| chr7:99,580,108–99,580,792 | 141.5 kb | Distal (>10kb) Multiome | 296 | |
| chr7:99,616,474–99,617,353 | 177.9 kb | Distal (>10kb) Multiome | 1043 | |
| chr7:99,693,257–99,693,867 | 254.6 kb | Distal (>10kb) Multiome | 518 |
Genomic view of the CPSF4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.