Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CLDN3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CLDN3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CLDN3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:73,521,449–73,522,945 | 247.8 kb | Distal (>10kb) Multiome | 977 | |
| chr7:73,556,852–73,558,145 | 212.9 kb | Distal (>10kb) Multiome | 1080 | |
| chr7:73,578,111–73,579,357 | 191.5 kb | Distal (>10kb) Multiome | 765 | |
| chr7:73,612,552–73,613,365 | 157.3 kb | Distal (>10kb) Multiome | 733 | |
| chr7:73,622,785–73,623,555 | 147.1 kb | Distal (>10kb) Multiome | 659 | |
| chr7:73,623,956–73,624,915 | 145.7 kb | Distal (>10kb) Multiome | 652 | |
| chr7:73,647,765–73,648,318 | 122.3 kb | Distal (>10kb) Multiome | 506 | |
| chr7:73,667,449–73,668,409 | 102.5 kb | Distal (>10kb) Multiome | 678 | |
| chr7:73,682,807–73,684,039 | 86.8 kb | Distal (>10kb) Multiome | 803 | |
| chr7:73,703,674–73,704,431 | 66.2 kb | Distal (>10kb) Multiome | 226 | |
| chr7:73,719,255–73,720,191 | 50.6 kb | Distal (>10kb) Multiome | 478 | |
| chr7:73,734,760–73,735,432 | 35.4 kb | Distal (>10kb) Multiome | 592 | |
| chr7:73,738,048–73,739,612 | 31.3 kb | Distal (>10kb) Multiome | 949 | |
| chr7:73,741,460–73,743,811 | 27.2 kb | Distal (>10kb) Multiome | 604 | |
| chr7:73,769,598–73,771,029 | 188 bp | At TSS Multiome | 496 | |
| chr7:73,831,659–73,832,518 | 62.0 kb | Distal (>10kb) Multiome | 447 | |
| chr7:73,841,730–73,843,136 | 72.2 kb | Distal (>10kb) Multiome | 575 | |
| chr7:73,885,944–73,886,823 | 116.1 kb | Distal (>10kb) Multiome | 480 | |
| chr7:74,026,698–74,027,309 | 256.7 kb | Distal (>10kb) Multiome | 294 | |
| chr7:74,067,853–74,068,319 | 297.8 kb | Distal (>10kb) Multiome | 359 |
Genomic view of the CLDN3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.