Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CKMT2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CKMT2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CKMT2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:80,957,092–80,957,694 | 276.0 kb | Distal (>10kb) Multiome | 159 | |
| chr5:80,960,023–80,961,334 | 272.9 kb | Distal (>10kb) Multiome | 603 | |
| chr5:81,232,714–81,233,670 | 20 bp | At TSS Multiome | 329 | |
| chr5:81,234,241–81,234,545 | 922 bp | At TSS | 14 | |
| chr5:81,234,810–81,235,316 | 1.5 kb | Proximal (<10kb) | 81 | |
| chr5:81,269,902–81,270,952 | 37.2 kb | Distal (>10kb) Multiome | 126 | |
| chr5:81,301,217–81,302,258 | 68.3 kb | Distal (>10kb) Multiome | 872 | |
| chr5:81,393,587–81,394,731 | 160.8 kb | Distal (>10kb) Multiome | 292 | |
| chr5:81,407,752–81,408,374 | 174.7 kb | Distal (>10kb) Multiome | 76 |
Genomic view of the CKMT2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.