This gene encodes a member of the chondroitin N-acetylgalactosaminyltransferase family. These enzymes possess dual glucuronyltransferase and galactosaminyltransferase activity and play critical roles in the biosynthesis of chondroitin sulfate, a glycosaminoglycan involved in many biological processes including cell proliferation and morphogenesis. Decreased expression of this gene may play a role in colorectal cancer, and mutations in this gene are a cause of temtamy preaxial brachydactyly syndrome. [provided by RefSeq, Dec 2011]
Transcription factors with Perturb-seq knockdown data for CHSY1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHSY1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHSY1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:100,917,841–100,918,691 | 333.9 kb | Distal (>10kb) Multiome HiCAR | 405 | |
| chr15:100,918,843–100,920,172 | 332.6 kb | Distal (>10kb) Multiome HiCAR | 364 | |
| chr15:101,008,268–101,008,757 | 243.5 kb | Distal (>10kb) Multiome HiCAR | 571 | |
| chr15:101,068,707–101,069,646 | 182.8 kb | Distal (>10kb) Multiome HiCAR | 435 | |
| chr15:101,077,927–101,078,550 | 173.9 kb | Distal (>10kb) Multiome HiCAR | 35 | |
| chr15:101,088,678–101,089,262 | 163.1 kb | Distal (>10kb) Multiome HiCAR | 541 | |
| chr15:101,201,405–101,201,942 | 50.4 kb | Distal (>10kb) Multiome | 276 | |
| chr15:101,250,886–101,252,530 | 17 bp | At TSS Multiome | 729 | |
| chr15:101,258,024–101,258,414 | 6.0 kb | Proximal (<10kb) | 196 | |
| chr15:101,276,817–101,277,913 | 25.5 kb | Distal (>10kb) Multiome | 748 | |
| chr15:101,294,732–101,295,714 | 43.2 kb | Distal (>10kb) Multiome | 987 | |
| chr15:101,489,250–101,490,332 | 237.6 kb | Distal (>10kb) Multiome | 422 |
Genomic view of the CHSY1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.