This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009]
Transcription factors with Perturb-seq knockdown data for CHST4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHST4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHST4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:71,230,392–71,230,982 | 295.8 kb | Distal (>10kb) Multiome | 194 | |
| chr16:71,288,904–71,289,782 | 237.0 kb | Distal (>10kb) Multiome | 1067 | |
| chr16:71,358,510–71,359,053 | 167.7 kb | Distal (>10kb) Multiome | 387 | |
| chr16:71,412,167–71,413,209 | 113.8 kb | Distal (>10kb) Multiome | 349 | |
| chr16:71,425,887–71,427,162 | 99.9 kb | Distal (>10kb) Multiome | 924 | |
| chr16:71,441,362–71,442,080 | 84.7 kb | Distal (>10kb) Multiome | 232 | |
| chr16:71,461,920–71,462,398 | 64.2 kb | Distal (>10kb) Multiome | 944 | |
| chr16:71,484,066–71,484,742 | 42.0 kb | Distal (>10kb) Multiome | 801 | |
| chr16:71,526,040–71,526,860 | 16 bp | At TSS Multiome | 294 | |
| chr16:71,564,628–71,565,649 | 38.7 kb | Distal (>10kb) Multiome | 792 | |
| chr16:71,625,835–71,627,289 | 100.6 kb | Distal (>10kb) Multiome | 594 | |
| chr16:71,722,829–71,724,442 | 197.5 kb | Distal (>10kb) Multiome | 894 | |
| chr16:71,808,251–71,809,603 | 282.7 kb | Distal (>10kb) Multiome | 1032 |
Genomic view of the CHST4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.