This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.[provided by RefSeq, Oct 2009]
Transcription factors with Perturb-seq knockdown data for CHMP4B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHMP4B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHMP4B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:33,663,243–33,664,235 | 147.6 kb | Distal (>10kb) Multiome | 895 | |
| chr20:33,666,358–33,668,405 | 144.5 kb | Distal (>10kb) Multiome | 603 | |
| chr20:33,673,732–33,675,136 | 136.9 kb | Distal (>10kb) Multiome | 826 | |
| chr20:33,685,755–33,686,963 | 124.8 kb | Distal (>10kb) Multiome | 830 | |
| chr20:33,720,042–33,720,684 | 91.0 kb | Distal (>10kb) Multiome | 584 | |
| chr20:33,731,496–33,732,906 | 79.2 kb | Distal (>10kb) Multiome | 1049 | |
| chr20:33,811,016–33,811,951 | 82 bp | At TSS Multiome | 921 | |
| chr20:33,992,591–33,994,776 | 182.1 kb | Distal (>10kb) Multiome | 1067 |
Genomic view of the CHMP4B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.