Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]
Transcription factors with Perturb-seq knockdown data for CHKB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHKB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHKB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:50,299,702–50,300,750 | 282.4 kb | Distal (>10kb) Multiome | 250 | |
| chr22:50,307,140–50,307,760 | 275.2 kb | Distal (>10kb) Multiome | 392 | |
| chr22:50,326,615–50,327,631 | 255.7 kb | Distal (>10kb) Multiome | 754 | |
| chr22:50,342,153–50,342,608 | 240.3 kb | Distal (>10kb) Multiome | 553 | |
| chr22:50,342,772–50,343,603 | 239.6 kb | Distal (>10kb) Multiome | 573 | |
| chr22:50,474,348–50,475,571 | 107.7 kb | Distal (>10kb) Multiome | 487 | |
| chr22:50,481,062–50,482,150 | 101.3 kb | Distal (>10kb) Multiome | 387 | |
| chr22:50,506,343–50,508,710 | 74.8 kb | Distal (>10kb) Multiome | 856 | |
| chr22:50,524,965–50,527,480 | 55.7 kb | Distal (>10kb) Multiome | 1031 | |
| chr22:50,529,065–50,532,692 | 51.9 kb | Distal (>10kb) Multiome | 745 | |
| chr22:50,562,609–50,563,508 | 19.9 kb | Distal (>10kb) Multiome | 680 | |
| chr22:50,582,134–50,583,431 | 103 bp | At TSS Multiome | 871 | |
| chr22:50,627,527–50,628,705 | 45.4 kb | Distal (>10kb) Multiome | 685 | |
| chr22:50,674,352–50,675,344 | 92.2 kb | Distal (>10kb) Multiome | 386 | |
| chr22:50,696,725–50,697,564 | 114.6 kb | Distal (>10kb) Multiome | 184 | |
| chr22:50,720,392–50,721,059 | 137.7 kb | Distal (>10kb) Multiome | 60 |
Genomic view of the CHKB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.