The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPT is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for CENPT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CENPT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CENPT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:67,537,215–67,538,620 | 295.8 kb | Distal (>10kb) Multiome | 560 | |
| chr16:67,561,597–67,563,672 | 271.6 kb | Distal (>10kb) Multiome | 1044 | |
| chr16:67,644,719–67,645,304 | 188.9 kb | Distal (>10kb) Multiome | 638 | |
| chr16:67,649,353–67,649,999 | 184.2 kb | Distal (>10kb) Multiome | 112 | |
| chr16:67,652,610–67,653,810 | 180.9 kb | Distal (>10kb) Multiome | 498 | |
| chr16:67,659,631–67,661,707 | 173.1 kb | Distal (>10kb) Multiome | 962 | |
| chr16:67,666,180–67,667,235 | 167.3 kb | Distal (>10kb) Multiome | 713 | |
| chr16:67,667,674–67,668,349 | 165.8 kb | Distal (>10kb) Multiome | 628 | |
| chr16:67,718,772–67,720,138 | 114.6 kb | Distal (>10kb) Multiome | 866 | |
| chr16:67,806,066–67,807,386 | 27.1 kb | Distal (>10kb) Multiome | 914 | |
| chr16:67,816,404–67,816,888 | 17.1 kb | Distal (>10kb) Multiome | 690 | |
| chr16:67,833,426–67,834,269 | 1 bp | At TSS Multiome | 838 | |
| chr16:67,841,337–67,843,276 | 8.2 kb | Proximal (<10kb) Multiome | 927 | |
| chr16:67,846,090–67,847,897 | 12.9 kb | Distal (>10kb) Multiome | 1025 | |
| chr16:67,872,739–67,873,665 | 39.2 kb | Distal (>10kb) Multiome | 982 | |
| chr16:67,884,566–67,885,097 | 51.0 kb | Distal (>10kb) Multiome | 264 | |
| chr16:67,892,898–67,893,885 | 59.3 kb | Distal (>10kb) Multiome | 867 | |
| chr16:67,915,108–67,915,887 | 81.5 kb | Distal (>10kb) Multiome | 92 | |
| chr16:67,935,189–67,936,596 | 101.9 kb | Distal (>10kb) Multiome | 980 | |
| chr16:67,963,746–67,964,209 | 130.1 kb | Distal (>10kb) Multiome | 161 | |
| chr16:67,968,087–67,969,341 | 134.8 kb | Distal (>10kb) Multiome | 861 | |
| chr16:67,993,130–67,993,736 | 159.5 kb | Distal (>10kb) Multiome | 790 | |
| chr16:68,019,222–68,020,043 | 185.6 kb | Distal (>10kb) Multiome | 108 | |
| chr16:68,022,490–68,023,556 | 189.3 kb | Distal (>10kb) Multiome | 958 | |
| chr16:68,084,205–68,086,133 | 251.3 kb | Distal (>10kb) Multiome | 1092 |
Genomic view of the CENPT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.