This gene encodes a component of the interphase centromere complex. The encoded protein is localized to the centromere throughout the cell cycle and is required for bipolar spindle assembly, chromosome segregation and checkpoint signaling during mitosis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
Transcription factors with Perturb-seq knockdown data for CENPO. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CENPO upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CENPO, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:24,456,964–24,457,548 | 335.9 kb | Distal (>10kb) Multiome HiCAR | 112 | |
| chr2:24,489,608–24,492,569 | 302.1 kb | Distal (>10kb) Multiome HiCAR | 854 | |
| chr2:24,791,115–24,791,348 | 1.8 kb | Proximal (<10kb) | 167 | |
| chr2:24,792,642–24,794,076 | 165 bp | At TSS Multiome | 1010 | |
| chr2:24,821,671–24,822,465 | 29.1 kb | Distal (>10kb) Multiome | 172 | |
| chr2:24,827,474–24,828,282 | 34.7 kb | Distal (>10kb) Multiome | 460 | |
| chr2:24,919,504–24,920,824 | 127.1 kb | Distal (>10kb) Multiome | 431 | |
| chr2:24,971,247–24,972,384 | 178.8 kb | Distal (>10kb) Multiome | 1032 | |
| chr2:25,041,314–25,043,455 | 248.9 kb | Distal (>10kb) Multiome | 743 |
Genomic view of the CENPO locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.