This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]
Transcription factors with Perturb-seq knockdown data for CDK8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CDK8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CDK8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr13:25,977,016–25,977,600 | 276.8 kb | Distal (>10kb) Multiome | 163 | |
| chr13:25,977,740–25,978,960 | 275.5 kb | Distal (>10kb) Multiome | 191 | |
| chr13:26,050,068–26,052,550 | 202.0 kb | Distal (>10kb) Multiome | 468 | |
| chr13:26,186,100–26,186,936 | 67.5 kb | Distal (>10kb) Multiome | 821 | |
| chr13:26,221,461–26,222,628 | 32.2 kb | Distal (>10kb) Multiome | 979 | |
| chr13:26,253,747–26,254,977 | 9 bp | At TSS Multiome | 742 | |
| chr13:26,475,352–26,476,527 | 221.7 kb | Distal (>10kb) Multiome | 241 |
Genomic view of the CDK8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.