The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CDK4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CDK4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CDK4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:57,454,516–57,456,639 | 296.0 kb | Distal (>10kb) Multiome | 841 | |
| chr12:57,458,743–57,463,318 | 291.0 kb | Distal (>10kb) Multiome | 994 | |
| chr12:57,475,229–57,476,151 | 276.8 kb | Distal (>10kb) Multiome | 194 | |
| chr12:57,487,577–57,488,336 | 264.4 kb | Distal (>10kb) Multiome | 784 | |
| chr12:57,519,488–57,521,440 | 231.7 kb | Distal (>10kb) Multiome | 1029 | |
| chr12:57,521,541–57,523,658 | 229.7 kb | Distal (>10kb) Multiome | 960 | |
| chr12:57,546,771–57,547,419 | 205.1 kb | Distal (>10kb) Multiome | 623 | |
| chr12:57,549,654–57,550,343 | 202.3 kb | Distal (>10kb) Multiome | 366 | |
| chr12:57,583,383–57,583,948 | 168.8 kb | Distal (>10kb) Multiome | 353 | |
| chr12:57,590,813–57,591,877 | 161.2 kb | Distal (>10kb) Multiome | 738 | |
| chr12:57,604,466–57,605,404 | 147.5 kb | Distal (>10kb) Multiome HiCAR | 337 | |
| chr12:57,609,052–57,609,957 | 143.0 kb | Distal (>10kb) Multiome HiCAR | 83 | |
| chr12:57,610,841–57,611,876 | 140.9 kb | Distal (>10kb) Multiome | 558 | |
| chr12:57,621,603–57,622,199 | 130.5 kb | Distal (>10kb) Multiome | 446 | |
| chr12:57,631,837–57,633,369 | 119.2 kb | Distal (>10kb) Multiome | 706 | |
| chr12:57,693,486–57,694,647 | 58.4 kb | Distal (>10kb) Multiome | 757 | |
| chr12:57,726,079–57,726,879 | 26.1 kb | Distal (>10kb) Multiome | 597 | |
| chr12:57,738,612–57,739,176 | 13.3 kb | Distal (>10kb) Multiome | 201 | |
| chr12:57,742,146–57,742,369 | 10.0 kb | Proximal (<10kb) | 170 | |
| chr12:57,744,434–57,745,735 | 7.4 kb | Proximal (<10kb) Multiome HiCAR | 822 | |
| chr12:57,751,806–57,753,147 | 131 bp | At TSS Multiome | 1030 | |
| chr12:57,754,455–57,756,188 | 2.6 kb | Proximal (<10kb) Multiome | 616 | |
| chr12:57,756,649–57,757,193 | 4.6 kb | Proximal (<10kb) Multiome | 315 | |
| chr12:57,765,020–57,765,720 | 13.0 kb | Distal (>10kb) Multiome HiCAR | 472 | |
| chr12:57,766,452–57,767,514 | 14.7 kb | Distal (>10kb) Multiome HiCAR | 330 | |
| chr12:57,771,528–57,773,018 | 19.8 kb | Distal (>10kb) Multiome | 1006 | |
| chr12:57,782,579–57,783,204 | 30.4 kb | Distal (>10kb) Multiome | 916 | |
| chr12:57,814,980–57,815,520 | 63.0 kb | Distal (>10kb) Multiome HiCAR | 117 | |
| chr12:57,844,422–57,847,797 | 94.7 kb | Distal (>10kb) Multiome | 1032 | |
| chr12:57,852,281–57,853,469 | 100.5 kb | Distal (>10kb) Multiome | 295 | |
| chr12:57,865,060–57,865,767 | 113.1 kb | Distal (>10kb) Multiome | 547 | |
| chr12:57,888,522–57,889,064 | 136.3 kb | Distal (>10kb) Multiome | 328 | |
| chr12:57,896,473–57,897,915 | 144.6 kb | Distal (>10kb) Multiome | 419 | |
| chr12:57,935,860–57,936,461 | 183.8 kb | Distal (>10kb) Multiome | 650 | |
| chr12:57,940,778–57,942,227 | 189.2 kb | Distal (>10kb) Multiome | 836 | |
| chr12:59,328,376–59,329,451 | 1576.4 kb | Distal (>10kb) Multiome HiCAR | 131 |
Genomic view of the CDK4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.