The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]
Transcription factors with Perturb-seq knockdown data for CDC27. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CDC27 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CDC27, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:46,922,775–46,923,450 | 266.2 kb | Distal (>10kb) Multiome | 986 | |
| chr17:47,099,846–47,100,680 | 89.0 kb | Distal (>10kb) Multiome | 574 | |
| chr17:47,188,797–47,189,938 | 132 bp | At TSS Multiome | 1050 | |
| chr17:47,253,593–47,254,400 | 64.7 kb | Distal (>10kb) Multiome | 571 | |
| chr17:47,275,398–47,275,943 | 86.4 kb | Distal (>10kb) Multiome | 340 | |
| chr17:47,310,107–47,310,844 | 121.2 kb | Distal (>10kb) Multiome | 92 | |
| chr17:47,323,422–47,324,660 | 134.7 kb | Distal (>10kb) Multiome | 1009 | |
| chr17:47,423,404–47,423,999 | 234.5 kb | Distal (>10kb) Multiome | 129 |
Genomic view of the CDC27 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.