CCDC66
coiled-coil domain containing 66 | DKFZp686C0433

Enables microtubule binding activity. Involved in several processes, including ciliary transition zone assembly; microtubule cytoskeleton organization; and mitotic metaphase chromosome alignment. Located in several cellular components, including Flemming body; cytoskeleton; and photoreceptor inner segment. [provided by Alliance of Genome Resources, Jul 2025]

Member of: DE-2 Developmental clusters: GC5
Biological processes 46 terms
axoneme (GO:0005930)axoneme (GO:0005930)centriolar satellite (GO:0034451)centriolar satellite (GO:0034451)centrosome (GO:0005813)centrosome (GO:0005813)ciliary basal body (GO:0036064)ciliary transition zone (GO:0035869)ciliary transition zone assembly (GO:1905349)cilium (GO:0005929)cilium (GO:0005929)cilium (GO:0005929)cilium assembly (GO:0060271)cilium assembly (GO:0060271)cilium disassembly (GO:0061523)cilium disassembly (GO:0061523)detection of light stimulus involved in visual perception (GO:0050908)establishment of mitotic spindle orientation (GO:0000132)identical protein binding (GO:0042802)microtubule (GO:0005874)microtubule (GO:0005874)microtubule binding (GO:0008017)microtubule binding (GO:0008017)microtubule bundle formation (GO:0001578)microtubule bundle formation (GO:0001578)microtubule cytoskeleton organization involved in mitosis (GO:1902850)microtubule nucleation (GO:0007020)midbody (GO:0030496)midbody (GO:0030496)mitotic metaphase chromosome alignment (GO:0007080)mitotic spindle assembly (GO:0090307)mitotic spindle organization (GO:0007052)photoreceptor inner segment (GO:0001917)photoreceptor inner segment (GO:0001917)photoreceptor inner segment (GO:0001917)photoreceptor outer segment (GO:0001750)positive regulation of smoothened signaling pathway (GO:0045880)positive regulation of smoothened signaling pathway (GO:0045880)protein binding (GO:0005515)protein homodimerization activity (GO:0042803)protein homodimerization activity (GO:0042803)regulation of cytokinesis (GO:0032465)regulation of protein localization to cilium (GO:1903564)retina homeostasis (GO:0001895)spindle (GO:0005819)spindle (GO:0005819)
Expression (TPM)
CCDC66 — as a Regulated Gene

TFs regulating CCDC66 0 TFs

Transcription factors with Perturb-seq knockdown data for CCDC66. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CCDC66 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to CCDC66

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CCDC66, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr3:56,467,084–56,467,577 90.0 kb Distal (>10kb) Multiome 156
chr3:56,467,706–56,468,963 88.6 kb Distal (>10kb) Multiome 376
chr3:56,556,857–56,557,974 8 bp At TSS Multiome 756
chr3:56,682,322–56,683,608 126.0 kb Distal (>10kb) Multiome 963
chr3:56,782,366–56,782,841 225.5 kb Distal (>10kb) Multiome 265
chr3:56,801,406–56,802,556 244.9 kb Distal (>10kb) Multiome 717
chr3:56,823,361–56,824,198 266.5 kb Distal (>10kb) Multiome 160

Genome Browser

Genomic view of the CCDC66 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr3:56,457,084 – 56,834,198
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq