The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Transcription factors with Perturb-seq knockdown data for CARD8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CARD8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CARD8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:48,169,480–48,171,282 | 33.5 kb | Distal (>10kb) Multiome | 950 | |
| chr19:48,203,709–48,204,551 | 85 bp | At TSS Multiome | 741 | |
| chr19:48,271,198–48,272,050 | 67.6 kb | Distal (>10kb) Multiome | 562 | |
| chr19:48,290,181–48,291,716 | 86.9 kb | Distal (>10kb) Multiome | 666 | |
| chr19:48,320,062–48,322,328 | 117.5 kb | Distal (>10kb) Multiome | 826 | |
| chr19:48,330,094–48,330,787 | 126.4 kb | Distal (>10kb) Multiome | 460 | |
| chr19:48,332,701–48,333,506 | 129.0 kb | Distal (>10kb) Multiome | 615 | |
| chr19:48,333,584–48,334,372 | 130.2 kb | Distal (>10kb) Multiome | 554 | |
| chr19:48,363,062–48,364,710 | 160.3 kb | Distal (>10kb) Multiome | 940 | |
| chr19:48,390,663–48,391,935 | 187.6 kb | Distal (>10kb) Multiome | 816 | |
| chr19:48,397,916–48,399,301 | 194.9 kb | Distal (>10kb) Multiome | 619 | |
| chr19:48,399,411–48,400,900 | 196.6 kb | Distal (>10kb) Multiome | 548 | |
| chr19:48,414,121–48,416,085 | 211.6 kb | Distal (>10kb) Multiome | 298 | |
| chr19:48,445,379–48,446,456 | 241.9 kb | Distal (>10kb) Multiome | 845 | |
| chr19:48,468,758–48,470,027 | 265.2 kb | Distal (>10kb) Multiome | 860 | |
| chr19:48,490,661–48,494,449 | 287.4 kb | Distal (>10kb) Multiome | 633 | |
| chr19:48,500,898–48,503,050 | 298.1 kb | Distal (>10kb) Multiome | 656 |
Genomic view of the CARD8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.