The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Transcription factors with Perturb-seq knockdown data for CAPN2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CAPN2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CAPN2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:223,129,163–223,129,910 | 583.0 kb | Distal (>10kb) Multiome HiCAR | 411 | |
| chr1:223,710,572–223,710,872 | 1.7 kb | Proximal (<10kb) | 83 | |
| chr1:223,711,902–223,713,324 | 36 bp | At TSS Multiome | 708 | |
| chr1:223,715,408–223,715,608 | 2.9 kb | Proximal (<10kb) | 364 | |
| chr1:223,748,526–223,749,571 | 36.4 kb | Distal (>10kb) Multiome HiCAR | 391 | |
| chr1:223,845,336–223,846,577 | 133.5 kb | Distal (>10kb) Multiome | 1001 | |
| chr1:223,870,523–223,871,422 | 158.3 kb | Distal (>10kb) Multiome | 131 | |
| chr1:223,992,339–223,993,214 | 280.1 kb | Distal (>10kb) Multiome HiCAR | 350 | |
| chr1:224,329,851–224,330,705 | 617.8 kb | Distal (>10kb) Multiome HiCAR | 865 |
Genomic view of the CAPN2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.