The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Transcription factors with Perturb-seq knockdown data for CAPN1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CAPN1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CAPN1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:64,887,785–64,888,374 | 293.8 kb | Distal (>10kb) Multiome | 633 | |
| chr11:64,916,057–64,917,940 | 264.4 kb | Distal (>10kb) Multiome | 873 | |
| chr11:64,924,041–64,924,916 | 257.4 kb | Distal (>10kb) Multiome | 808 | |
| chr11:64,971,835–64,972,454 | 209.7 kb | Distal (>10kb) Multiome | 265 | |
| chr11:65,013,538–65,014,838 | 167.8 kb | Distal (>10kb) Multiome | 980 | |
| chr11:65,027,243–65,027,944 | 154.2 kb | Distal (>10kb) Multiome | 851 | |
| chr11:65,040,595–65,041,884 | 140.7 kb | Distal (>10kb) Multiome | 838 | |
| chr11:65,083,636–65,084,788 | 97.6 kb | Distal (>10kb) Multiome | 898 | |
| chr11:65,095,834–65,097,052 | 85.3 kb | Distal (>10kb) Multiome | 1061 | |
| chr11:65,111,466–65,113,404 | 70.0 kb | Distal (>10kb) Multiome | 919 | |
| chr11:65,116,528–65,118,298 | 64.0 kb | Distal (>10kb) Multiome | 973 | |
| chr11:65,121,474–65,122,848 | 59.5 kb | Distal (>10kb) Multiome | 817 | |
| chr11:65,134,120–65,135,530 | 46.8 kb | Distal (>10kb) Multiome | 997 | |
| chr11:65,180,773–65,182,676 | 134 bp | At TSS Multiome | 832 | |
| chr11:65,184,109–65,184,542 | 2.6 kb | Proximal (<10kb) Multiome | 85 | |
| chr11:65,213,383–65,213,853 | 31.9 kb | Distal (>10kb) Multiome | 174 | |
| chr11:65,225,439–65,226,037 | 43.8 kb | Distal (>10kb) Multiome | 522 | |
| chr11:65,261,186–65,262,526 | 80.0 kb | Distal (>10kb) Multiome | 777 | |
| chr11:65,314,302–65,316,053 | 133.9 kb | Distal (>10kb) Multiome | 803 | |
| chr11:65,333,360–65,334,372 | 152.1 kb | Distal (>10kb) Multiome | 654 | |
| chr11:65,354,023–65,355,987 | 172.9 kb | Distal (>10kb) Multiome | 677 | |
| chr11:65,366,306–65,366,977 | 184.8 kb | Distal (>10kb) Multiome | 309 | |
| chr11:65,381,835–65,382,758 | 200.4 kb | Distal (>10kb) Multiome | 984 | |
| chr11:65,386,320–65,387,097 | 204.8 kb | Distal (>10kb) Multiome | 757 | |
| chr11:65,416,383–65,417,015 | 234.7 kb | Distal (>10kb) Multiome | 664 | |
| chr11:65,417,855–65,419,687 | 237.3 kb | Distal (>10kb) Multiome | 1018 | |
| chr11:65,421,858–65,423,533 | 240.6 kb | Distal (>10kb) Multiome | 1197 | |
| chr11:65,454,735–65,455,360 | 273.4 kb | Distal (>10kb) Multiome | 573 | |
| chr11:65,477,100–65,477,990 | 295.8 kb | Distal (>10kb) Multiome | 991 |
Genomic view of the CAPN1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.