Transcription factors with Perturb-seq knockdown data for C8orf44. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C8orf44 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C8orf44, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:66,428,113–66,430,401 | 238.8 kb | Distal (>10kb) Multiome | 921 | |
| chr8:66,431,895–66,432,840 | 235.1 kb | Distal (>10kb) Multiome | 609 | |
| chr8:66,438,980–66,439,800 | 228.4 kb | Distal (>10kb) Multiome | 484 | |
| chr8:66,512,582–66,513,139 | 154.8 kb | Distal (>10kb) Multiome | 239 | |
| chr8:66,612,352–66,614,219 | 54.2 kb | Distal (>10kb) Multiome | 862 | |
| chr8:66,666,261–66,667,884 | 263 bp | At TSS Multiome | 889 | |
| chr8:66,712,294–66,713,281 | 45.1 kb | Distal (>10kb) Multiome | 648 | |
| chr8:66,774,026–66,775,860 | 107.5 kb | Distal (>10kb) Multiome | 957 | |
| chr8:66,870,495–66,871,236 | 203.2 kb | Distal (>10kb) Multiome | 352 | |
| chr8:66,924,535–66,926,136 | 258.0 kb | Distal (>10kb) Multiome | 998 | |
| chr8:66,961,090–66,963,532 | 295.0 kb | Distal (>10kb) Multiome | 438 | |
| chr8:67,028,419–67,029,071 | 361.0 kb | Distal (>10kb) Multiome HiCAR | 488 |
Genomic view of the C8orf44 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.