Transcription factors with Perturb-seq knockdown data for C20orf96. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C20orf96 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C20orf96, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:266,689–267,291 | 23.7 kb | Distal (>10kb) Multiome | 931 | |
| chr20:290,009–290,864 | 227 bp | At TSS Multiome | 706 | |
| chr20:295,883–296,330 | 5.5 kb | Proximal (<10kb) | 27 | |
| chr20:297,159–298,781 | 6.7 kb | Proximal (<10kb) Multiome | 1084 | |
| chr20:300,328–300,910 | 9.9 kb | Proximal (<10kb) | 166 | |
| chr20:300,984–301,434 | 10.5 kb | Distal (>10kb) Multiome | 252 | |
| chr20:309,597–310,812 | 19.8 kb | Distal (>10kb) Multiome | 589 | |
| chr20:323,634–328,069 | 36.5 kb | Distal (>10kb) Multiome | 1260 | |
| chr20:330,299–330,873 | 39.9 kb | Distal (>10kb) Multiome | 376 | |
| chr20:346,592–348,332 | 57.2 kb | Distal (>10kb) Multiome | 1061 | |
| chr20:354,335–354,944 | 63.9 kb | Distal (>10kb) Multiome | 631 | |
| chr20:380,569–381,493 | 90.2 kb | Distal (>10kb) Multiome | 958 | |
| chr20:381,625–382,263 | 91.3 kb | Distal (>10kb) Multiome | 745 | |
| chr20:407,136–409,109 | 117.3 kb | Distal (>10kb) Multiome | 1064 | |
| chr20:461,861–463,102 | 171.8 kb | Distal (>10kb) Multiome | 871 | |
| chr20:543,069–544,398 | 253.1 kb | Distal (>10kb) Multiome | 965 | |
| chr20:555,914–556,523 | 265.4 kb | Distal (>10kb) Multiome | 249 | |
| chr20:574,398–574,909 | 283.9 kb | Distal (>10kb) Multiome | 119 |
Genomic view of the C20orf96 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.